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KMID : 0545120070170020359
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 2 p.359 ~ p.363
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Interaction Proteome Analysis of Xanthomonas Hrp Proteins
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Jang MI
Lee Doo-Hee
Bae Kwang-Hee
Park Sung-Goo
Cho Sa-Yeon
Park Hyun-Seok
Park Byoung-Chul
Lee Baek-Rak
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Abstract
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Because of the importance of the type III proteinsecretion system in bacteria-plant interaction, its function in bacterial pathogenesis of plants has been intensively studied. To identify bacterial proteins interacting with Xanthomonas hrp gene products that are involved in pathogenicity, we performed the glutathione-bead binding analysis of Xanthomonas lysates containing GST-tagged Hrp proteins. Analysis of glutathione-bead bound proteins by 1-DE and MALDI-TOF has demonstrated that Avr proteins, RecA, and several components of the type III secretion system interact with HrpB protein. This proteomic approach could provide a powerful tool in finding interaction partners of Hrp proteins whose roles in host-pathogen interaction need further studies.
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KEYWORD
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hrp gene, interaction protein, 1-DE, MALDITOF, pathogenesis
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